Last updated: 2021-10-07

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Knit directory: QuRIE-seq_manuscript/

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Abstract

Current high-throughput single-cell multi-omics methods cannot concurrently map changes in (phospho)protein levels and the associated gene expression profiles. We present QuRIE-seq (Quantification of RNA and Intracellular Epitopes by sequencing) and use multi-factor omics analysis (MOFA+) to map signal transduction over multiple timescales. We demonstrate that QuRIE-seq can trace the activation of the B-cell receptor pathway at the minute and hour time-scale and provide insight into the mechanism of action of an inhibitory drug Ibrutinib.

In this repository

Three pages contain code to process, analyse and create figures from the QuRIE-seq manuscript:

Attribution

We are very thankful for the effords made by developers of MOFA+ and workflowr. These (well documented) R-packages enable respectively extensive multimodal data-analysis and reproducible code documentation.


The content in this repository is available under the CC BY 4.0 license.

For proper attribution, please cite our publication (Cell Reports Methods) containing description and analysis of all presented data and results.